Journal: Cell & Bioscience

Article Title: Tie2-expressing monocytes/macrophages promote angiogenesis in chronically ischaemic brain tissue

doi: 10.1186/s13578-025-01401-1

Figure Lengend Snippet: Results of MIR-ASL scanning and CD31 expression detection in the brains of nude mice. A Representative MRI-T2WI film showing the EMS surgical site (white arrow indicates the EMS) and MIR-ASL films showing differences in CBP between the various groups (white dashed lines indicate ROIs under the EMS surgical sites). B Column chart showing the perfusion ratios (EMS sides over non-EMS sides) of each group (n = 6, one-way ANOVA and Šídák's multiple comparisons test). C Representative western blot showing the relative expression of CD31 in the CIBT adjacent to the TM tissue in each group (normalized to β-actin expression). D Densitometric analyses of the relative expression of CD31 (n = 3, one-way ANOVA and Šídák's multiple comparisons test). E Representative confocal images showing the expression of CD31 in the CIBT in each group. Bar = 20 μm. F Column chart showing the counts of CD31 + cells in each group (n = 3, one-way ANOVA and Šídák's multiple comparisons test). The error bars represent the ± SDs. ASL: arterial spin labelling; CBP: cerebral blood perfusion; CIBT: chronically ischaemic brain tissue; EMS: encephalomyosynangiosis; MRI: magnetic resonance imaging; ROIs: regions of interest; TM: temporal muscle; T2WI: T2-weighted imaging

Article Snippet: The cell suspension was subsequently mixed with 5 μL of Annexin V-FITC staining solution and 10 μL of propidium iodide staining solution at room temperature in a dark room for 15 min. For direct coculture, anti-CD31 (10148-MM13-A, Sino Biological, China) was also used to label the HUVECs.

Techniques: Expressing, Western Blot, Magnetic Resonance Imaging, Imaging

Journal: Cell & Bioscience

Article Title: Tie2-expressing monocytes/macrophages promote angiogenesis in chronically ischaemic brain tissue

doi: 10.1186/s13578-025-01401-1

Figure Lengend Snippet: Immunofluorescence and western blotting results showing the expression of relevant cytokines in the CIBT of 2VO + EMS rats. A Representative triple immunofluorescence staining images showing ANGPT2 + , Tie2 + , and CD11b + cells in the CIBT in each group (white arrows indicate TEMs). Bar = 50 µm. B Counts of ANGPT2 + cells in each group (n = 3, one-way ANOVA and Šídák's multiple comparisons test). C Counts of TEMs (Tie2 + and CD11b + ) in each group (n = 9, one-way ANOVA and Šídák's multiple comparisons test). D Representative western blot showing the relative expression of TRPS1, ANGPT2, Tie2, CD11b, VEGFA, IGF1, and CD31 in the CIBT adjacent to the TM tissue in each group (normalized to β-actin expression). E Densitometric analyses of the relative expression of TRPS1, ANGPT2, Tie2, CD11b, VEGFA, IGF1, and CD31 (n = 3, one-way ANOVA and Šídák's multiple comparisons test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). The error bars represent the ± SDs. CIBT: chronically ischaemic brain tissue; EMS: encephalomyosynangiosis; TEMs: Tie2-expressing monocytes/macrophages; 2VO: 2-vessel occlusion

Article Snippet: The cell suspension was subsequently mixed with 5 μL of Annexin V-FITC staining solution and 10 μL of propidium iodide staining solution at room temperature in a dark room for 15 min. For direct coculture, anti-CD31 (10148-MM13-A, Sino Biological, China) was also used to label the HUVECs.

Techniques: Immunofluorescence, Western Blot, Expressing, Staining

Journal: Journal of Cellular and Molecular Medicine

Article Title: Directly auto-transplanted mesenchymal stem cells induce bone formation in a ceramic bone substitute in an ectopic sheep model

doi: 10.1111/j.1582-4934.2010.01131.x

Figure Lengend Snippet: Sheep MSC were characterized using FACS and RT-PCR analysis. (A) With RT-PCR analysis CD29, CD44 and CD166 expression of MSC could be proofed on mRNA level. As indicated by increased CD45 expression, ratio of hematopoietic cells was higher in directly auto-transplanted MSC as compared to expanded MSC. (B–D) FACS analysis revealed sheep MSC to express CD29, CD44 and CD166. Expanded MSC (B) were negative for the hematopoietic markers CD31 and CD45. Directly auto-transplanted cells (C) had a different expression pattern than expanded MSC. The directly auto-transplanted MSC had a weaker CD29 and CD166 but a stronger CD45 expression. Mean fluorescent indices are shown in (D).

Article Snippet: CD31 staining: After antigen retrieval with pH 6 solution (Target Retrieval Solution; Dako Cytomation) in a pressure cooker for 10 min. (Pascal; Dako Cytomation) peroxidase block (CSAII-System; Dako Cytomation) was applied for 15 min., followed by incubation with 10% goat serum (PromoCell GmbH) in PBS (PBS-Dulbecco 1×, Biochrom AG) for 30 min. and protein block with the CSA II-System for 30 min. Then sections were incubated with the primary monoclonal mouse anti-ovine antibody CD31 (Anti-CD31/PECAM-1, MorphoSys UK Ltd., Kidlington, Oxford, UK) at 1:100 diluted in 10% goat serum in PBS for 1 hr.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: Directly auto-transplanted mesenchymal stem cells induce bone formation in a ceramic bone substitute in an ectopic sheep model

doi: 10.1111/j.1582-4934.2010.01131.x

Figure Lengend Snippet: For determination of the cell type which is qualified best for bone tissue engineering purposes, different groups (expanded versus directly auto-transplanted MSC, groups 8–10) were investigated. In both groups cells were DiI labelled prior to implantation and implanted subcutaneously with or without BMP-2. (A–C) Expanded MSC (A), directly auto-transplanted MSC (B), BMP-2 in combination with directly auto-transplanted MSC (C). DiI-labelled MSC (red) could be found close to β-TCP/HA granules contributing to the newly formed bone parts. In the explants with directly auto-transplanted MSC a higher section of the DiI-labelled cells were found in the connective tissue parts of the constructs compared to the explants with expanded MSC or directly auto-transplanted MSC with BMP-2. (D–F) Sections of constructs of the groups with expanded MSC (D), directly auto-transplanted MSC (E), BMP-2 in combination with directly auto-transplanted MSC (F) were evaluated for vascularization. The constructs in all three groups are well vascularized as shown by CD31 immunohistochemistry (green). Nuclei are counterstained with DAPI (blue).

Article Snippet: CD31 staining: After antigen retrieval with pH 6 solution (Target Retrieval Solution; Dako Cytomation) in a pressure cooker for 10 min. (Pascal; Dako Cytomation) peroxidase block (CSAII-System; Dako Cytomation) was applied for 15 min., followed by incubation with 10% goat serum (PromoCell GmbH) in PBS (PBS-Dulbecco 1×, Biochrom AG) for 30 min. and protein block with the CSA II-System for 30 min. Then sections were incubated with the primary monoclonal mouse anti-ovine antibody CD31 (Anti-CD31/PECAM-1, MorphoSys UK Ltd., Kidlington, Oxford, UK) at 1:100 diluted in 10% goat serum in PBS for 1 hr.

Techniques: Construct, Immunohistochemistry

Journal: Interactive cardiovascular and thoracic surgery

Article Title: Epithelial grafting of a decellularized whole-tracheal segment: an in vivo experimental model.

doi: 10.1093/icvts/ivx442

Figure Lengend Snippet: Figure 4: (A) Implantation of an opened 2-cycle decellularized trachea in the lateral thoracic artery flap covered with a bilayered Integra template. (B) Buccal grafting was conducted after 21 days of revascularization. Buccal-graft ingrowth after 2 days (C), 5 days (D), 9 days (E) and 12 days (F). The superimposed cartilage rings are noted. Buccal graft adherence was confirmed via haematoxylin and eosin (G) and pan Cytokeratin-staining (H). CD31-staining showed the presence of endothelium- lined vessels (I). Non-grafted areas between buccal grafts also revascularized (black arrows) and re-epithelialized, as shown by haematoxylin and eosin (J) and pan Cytokeratin staining (K).

Article Snippet: Formalin-fixed samples were pretreated with citrate and incubated with pan Cytokeratin (1:50, pan Cytokeratin mouse anti-rabbit monoclonal antibody, Novus Biologicals, Littleton, CO, USA) for 30 min. Endothelium was visualized with CD31 incubation (1:50, CD31/PECAM1 mouse antirabbit monoclonal antibody, Novus Biologicals) for 30 min after pretreatment with Tris/ethylenediaminetetraacetic acid.

Techniques: Staining

Journal: Clinical Science (London, England : 1979)

Article Title: Adverse effects of ovarian cryopreservation and auto-transplantation on ovarian grafts and quality of produced oocytes in a mouse model

doi: 10.1042/CS20230483

Figure Lengend Snippet: ( A,B ) Immunohistochemistry for CD31. Green arrows indicate blood vessels with a diameter greater than 20 μm, red arrows indicate microvessels with a diameter less than 20 μm, and the scale bar = 20 μm. ( C ) The mean vascular density (MVD) of OTs. ( D– F ) Serum concentrations of FSH, E2, and AMH. Data are represented as means ± standard error. Individual data points are indicated by circles, triangles, and diamonds. The between group statistical significance ( P <0.05) is indicated by lower case letters, such that the bars labelled with a certain letter are not statistically different from each other but are statistically different from the bars without that letter. The fresh ovarian transplantation groups were designated as F7, F14, and F21, with collections on post-transplantation day 7, 14, and 21, respectively. The vitri-warmed ovarian groups were similarly named V7, V14, and V21. AMH, anti-Müllerian hormone; E2, estradiol; FSH, follicle-stimulating hormone; OT, ovarian tissue.

Article Snippet: Briefly, OT sections were treated with blocking solution and non-specific staining blockers (Maixin, Fujian, China) for 10 min sequentially after signal enhancement and cool down and subsequently incubated with anti-CD31 antibody (1:1000, Proteintech, Chicago, IL, U.S.A.) overnight at 4°C.

Techniques: Immunohistochemistry, Transplantation Assay

Journal: Stem Cell Research & Therapy

Article Title: Mesenchymal stem/stromal cells overexpressing leukemia inhibitory factor (LIF) promote arteriogenesis and functional recovery in a mouse model of critical hindlimb ischemia

doi: 10.1186/s13287-025-04762-z

Figure Lengend Snippet: Pro-angiogenic potential of MSC_LIF in vivo. A Matrigel plugs containing MSC or MSC_LIF conditioned medium or 0.9% saline solution (- CTL) after excision of the mouse ventral area ( n = 4 animals per group). B Quantification of hemoglobin (Hb) content inside the plugs using Drabkin’s reagent. C Indirect immunofluorescence of sections of paraffin-embedded Matrigel plugs labeled with antibodies against the endothelial marker CD31 (red) and the smooth muscle cell marker αSMA (green). Cell nuclei stained with DAPI (blue). Images obtained by laser confocal microscopy. Scale bars = 100 μm. D Quantification of the mean diameter of capillaries (CD31 + vessels) by area. E Quantification of the mean diameter of arterioles (vessels simultaneously CD31/ αSMA + ) by area. The one-way ANOVA test and the Bonferroni post-test were used to analyze statistical differences. Values expressed as mean ± SEM of two independent experiments. *** p < 0.001; ** p < 0.01

Article Snippet: Tissues were stained with goat anti-mouse CD31 antibody (R&D Systems, diluted 1:100 in 1% BSA Triton-X 0.3% solution) overnight at 4 °C, followed by incubation with Alexa FluorTM 568-conjugated chicken anti-goat antibody (Invitrogen - Thermo Fisher Scientific, diluted 1:1000 in 1x PBS), and Alexa FluorTM 488-conjugated rat anti-mouse αSMA antibody (eBioscience - Thermo Fisher Scientific, diluted 1:400 in 1x PBS) for 1 h at room temperature.

Techniques: In Vivo, Saline, Immunofluorescence, Labeling, Marker, Staining, Confocal Microscopy

Journal: Stem Cell Research & Therapy

Article Title: Mesenchymal stem/stromal cells overexpressing leukemia inhibitory factor (LIF) promote arteriogenesis and functional recovery in a mouse model of critical hindlimb ischemia

doi: 10.1186/s13287-025-04762-z

Figure Lengend Snippet: Assessment of blood vessel distribution in the GST muscle. A Indirect immunofluorescence of cryopreserved sections of GST muscle stained with antibodies against the endothelial marker CD31 (yellow) and the smooth muscle cell marker SMA (green). Cell nuclei were stained with DAPI (blue), and muscle cells were stained with phalloidin (red) ( n = 5 animals per group). Images obtained by laser confocal microscopy. Scale bars = 100 μm. B Quantification of mean capillary length/diameter (CD31 + vessels) per area. C Quantification of mean arteriole length/diameter (vessels simultaneously CD31/αSMA+). The Kruskal-Wallis test and the Dunn’s Multiple Comparison post-test were used to analyze statistical differences. Values expressed as mean ± SEM of three independent experiments. *** p < 0.001; ** p < 0.01; * p < 0.05. The NAIVE group corresponds to the non-induced paw

Article Snippet: Tissues were stained with goat anti-mouse CD31 antibody (R&D Systems, diluted 1:100 in 1% BSA Triton-X 0.3% solution) overnight at 4 °C, followed by incubation with Alexa FluorTM 568-conjugated chicken anti-goat antibody (Invitrogen - Thermo Fisher Scientific, diluted 1:1000 in 1x PBS), and Alexa FluorTM 488-conjugated rat anti-mouse αSMA antibody (eBioscience - Thermo Fisher Scientific, diluted 1:400 in 1x PBS) for 1 h at room temperature.

Techniques: Immunofluorescence, Staining, Marker, Confocal Microscopy, Comparison